Journal: Regenerative Biomaterials
Article Title: A bilayer bioengineered patch with sequential dual-growth factor release to promote vascularization in bladder reconstruction
doi: 10.1093/rb/rbae083
Figure Lengend Snippet: Effect of BSF scaffold on endothelial differentiation of ADSCs. ( A ) Q-PCR analysis of PECAM-1 gene expression on day 7, 14 and 21 ( n = 3). PECAM-1 gene expression in HUVECs was set as 1. ( B ) Q-PCR analysis of KDR gene expression on day 7, 14 and 21 ( n = 3). KDR gene expression in HUVECs was set as 1. ( C ) Immunofluorescence staining of CD31 on day 14. ( D ) Immunofluorescence staining of vWF on day 14. ( E ) Uptake of Ac-LDL and UEA-1 on day 14. ( F ) Number of cells positive for CD31 ( n = 3). ( G ) Number of cells positive for vWF ( n = 3). ( H ) Number of cells positive for Ac-LDL/UEA-1 double staining ( n = 3).
Article Snippet: After being washed twice with PBS, they were fixed in 4% paraformaldehyde for 15 min. Then they were incubated with 20 μg/ml of FITC-conjugated UEA-1 (Sigma Aldrich, USA) for 1 h at 37 °C.
Techniques: Expressing, Immunofluorescence, Staining, Double Staining